全文获取类型
收费全文 | 24887篇 |
免费 | 1766篇 |
国内免费 | 1745篇 |
专业分类
林业 | 1375篇 |
农学 | 1866篇 |
基础科学 | 317篇 |
6477篇 | |
综合类 | 9368篇 |
农作物 | 1596篇 |
水产渔业 | 1568篇 |
畜牧兽医 | 3701篇 |
园艺 | 1055篇 |
植物保护 | 1075篇 |
出版年
2024年 | 110篇 |
2023年 | 496篇 |
2022年 | 804篇 |
2021年 | 986篇 |
2020年 | 1022篇 |
2019年 | 1081篇 |
2018年 | 857篇 |
2017年 | 1307篇 |
2016年 | 1458篇 |
2015年 | 1115篇 |
2014年 | 1256篇 |
2013年 | 1774篇 |
2012年 | 2076篇 |
2011年 | 1913篇 |
2010年 | 1397篇 |
2009年 | 1404篇 |
2008年 | 1219篇 |
2007年 | 1408篇 |
2006年 | 1139篇 |
2005年 | 856篇 |
2004年 | 696篇 |
2003年 | 540篇 |
2002年 | 432篇 |
2001年 | 396篇 |
2000年 | 360篇 |
1999年 | 284篇 |
1998年 | 258篇 |
1997年 | 230篇 |
1996年 | 244篇 |
1995年 | 220篇 |
1994年 | 156篇 |
1993年 | 147篇 |
1992年 | 132篇 |
1991年 | 144篇 |
1990年 | 114篇 |
1989年 | 101篇 |
1988年 | 84篇 |
1987年 | 54篇 |
1986年 | 31篇 |
1985年 | 30篇 |
1984年 | 18篇 |
1983年 | 9篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1978年 | 3篇 |
1977年 | 4篇 |
1975年 | 3篇 |
1962年 | 5篇 |
1956年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
本研究通过对DNA提取裂解液的成分和用量以及裂解时间等因素进行系统优化,建立了一种高通量的磁珠法提取甘蔗总DNA,再进行PCR检测甘蔗杆状病毒(SCBV)的方法。采用核酸自动提取仪,应用纳米磁珠提取甘蔗总DNA,经PCR检测甘蔗杆状病毒,并与以试剂盒提取DNA为模板的PCR检测结果进行比较。结果显示,经优化的裂解液成分为EDTA·Na20.08 mol/L、NaCl 0.8 mol/L、SDS 2%和Tris-HCl0.10 mol/L,裂解时间为20 min,可用于纳米磁珠提取甘蔗总DNA进行SCBV检测。所建立的方法通量高、成本低、耗时短,可用于SCBV的批量检测。 相似文献
82.
83.
为研究叶面喷施水杨酸(SA)对不同苹果品种防御酶活性的影响,以抗病品种‘北之幸’和感病品种‘礼泉短富’为试验材料,对不同浓度水杨酸处理前后的苹果叶片进行防御酶活性测定。结果表明,经水杨酸处理后,‘北之幸’和‘礼泉短富’的β-1,3-葡聚糖酶、POD、PAL和PPO活性均有提高,且高于对照植株。其中‘北之幸’的β-1,3-葡聚糖酶、POD、PAL和PPO活性分别是对照的2.04倍,6.19倍,2.73倍和2.32倍;‘礼泉短富’的β-1,3-葡聚糖酶和PAL活性分别是对照的1.82倍和1.70倍,POD活性是对照的5.68倍,PPO活性是对照的4.00倍。由此可见,水杨酸处理可以不同程度的提高植株体内抗性相关酶的活性,但不同品种间存在差异。 相似文献
84.
马铃薯晚疫病的病原是致病疫霉菌(Phytophthora infestans),该病原菌在侵染马铃薯过程中分泌大量RxLR型效应子,但目前绝大多数RxLR效应子的功能和作用机制尚不明确。本研究成功克隆了致病疫霉菌的一个RxLR效应子PITG_16427.2,在本氏烟中瞬时表达PITG_16427.2,发现该效应子能够抑制6种激发子(INF1、PsojNIP、BAX、SIF2、Avh238、Avh241)激发的植物免疫反应。进一步研究发现,效应子PITG_16427.2在晚疫病菌侵染马铃薯早期上调表达。在15个致病疫霉菌株和3个同属菌株中克隆该基因,克隆到氨基酸序列一致性超过93%的同源基因,这些同源基因均能在本氏烟中抑制INF1和Avh241引起的HR,揭示了该效应子在病原卵菌中序列和功能的高度保守性。在本氏烟和马铃薯感病品种Désirée中瞬时表达PITG_16427.2,发现该效应子能够显著促进晚疫病菌的侵染。通过qRT-PCR方法检测发现,乙烯信号的相关基因ERF1显著上调,而水杨酸信号相关基因PR1b显著下调,表明PITG_16427.2在晚疫病菌侵染过程中可抑制寄主的SA信号途径,促进晚疫病菌侵染。因此,RxLR型效应子PITG_16427.2是致病疫霉菌中一个重要的侵染致病因子。 相似文献
85.
通过单因素试验(青枣果肉添加量、蔗糖添加量、奶粉添加量、接种量、发酵温度和发酵时间)研究青枣发酵饮料的感官品质,通过正交试验,确定台湾青枣发酵饮料的最佳配方为青枣果肉添加量30%,蔗糖添加量10%,奶粉添加量6%;青枣发酵乳酸饮料的最佳发酵工艺为发酵时间35 h,发酵温度30℃,接种量0.25%。 相似文献
86.
Meghnath Pokharel Anuj Chiluwal Michael Stamm Doohong Min Davina Rhodes S.V. Krishna Jagadish 《Journal of Agronomy and Crop Science》2020,206(5):579-596
Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod-filling stages. Although the impact of high day-time temperature stress on yield and quality has been documented in canola, similar information under high night-time temperature (HNT) stress is not available. Using six hybrids and four open-pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post-flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open-pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open-pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future. 相似文献
87.
为了研究不同火干扰烈度对喀纳斯泰加林土壤有机碳含量及碳储量的影响,以喀纳斯泰加林同一火烧时间不同火干扰烈度的火烧迹地为研究对象,对不同土层(0~10 cm,10~25 cm)有机碳含量进行测定并估算其碳储量,采用单因素方差分析及LSD多重比较进行差异显著性分析。结果表明,相同火干扰烈度下0~10 cm土层土壤有机碳及碳储量均大于10~25 cm土层。0~10 cm土层中碳储量表现为:未火烧>轻度火干扰>中度火干扰>重度火干扰,在10~25 cm土层中碳储量表现为:轻度火干扰>中度火干扰>未火烧>重度火干扰。0~10 cm土层的土壤有机碳含量和碳储量以及10~25 cm土层的有机碳含量损失程度会随着火干扰烈度的增加而增加。10~25 cm土层碳储量会在轻度和中度火干扰后出现增加。 相似文献
88.
89.
Xiao-feng YUE Yan-lun JU Zi-zhu TANG Ya-meng ZHAO Xu-liang JIAO Zhen-wen ZHANG 《农业科学学报》2019,18(9):2052-2062
The effects of the severity and timing of leaf removal(LR) on the amino acids of Sauvignon Blanc grapes and wines were studied during the 2017 growing season. High-performance liquid chromatography(HPLC) was used to analyze the amino acids profiles of grape berries and wines. The basal leaves were removed at three time points(40, 56 and 72 days after flowering, named LR40, LR56 and LR72, respectively) at two severity levels(one at which the first, third, and fifth basal leaves of each shoot were removed(50% level); and another at which the first six basal leaves were removed(100% level)). The results showed that leaf removal had little impact on total soluble solids(°Brix), titratable acidity, pH or berry weight. The LR72-50% treated grapes had higher berry weight, titratable acidity and °Brix than those of the other treatments. The highest concentrations of total amino acids and of total amino acids except proline were detected in LR72-50% treated grapes(2 952.58 and 2 764.36 mg L~(-1), respectively); the lowest were detected in LR72-100% treated grapes(2 172.82 and 2 038.71 mg L~(-1), respectively). LR72-50% treatment significantly promoted the synthesis of aspartic acid, serine, arginine, alanine, aminobutyric acid and proline at both severity levels for grapes, the concentrations of all of these amino acids were increased relative to the control concentrations. The LR72-50%, LR40-100% and LR72-100% treated wines had higher total amino acids concentrations and higher concentrations of some individual amino acids, such as arginine, alanine and serine, than did the control wines. Of all the amino acids studied, glycine, tyrosine, cysteine, methionine and lysine were not significantly influenced by the timing or severity basal defoliation in grapes and wines. The present study reveals the effects of the timing and severity of leaf removal on the amino acids profiles of grapes and wines. 相似文献
90.
LU Feng-zhong YU Hao-qiang LI Si LI Wan-chen ZHANG Zhi-yong FU Feng-ling 《农业科学学报》2020,19(9):2165-2176
Pyrabactin resistance 1-like proteins(PYLs) are direct receptors of abscisic acid(ABA). For the redundant and polymorphic functions, some members of the PYL family interact with components of other signaling pathways. Here, 253 positive colonies from a maize cDNA library were screened as interacting proteins with the members of ZmPYL family. After sequencing and function annotation, 17 of 28 interaction combinations were verified by yeast two-hybrid(Y2 H). The germination potential, taproot length and proline content of a quartet mutant of Arabidopsis PYL genes were significantly deceased comparing to the wild type(WT) under alkaline stress(pH 8.5) and 100 μmol L–1 methyl jasmonate(MeJA) induction. The malondialdehyde(MDA) content was significantly increased. After germinating in darkness, the characteristics of dark morphogenesis of the quartet mutant seedlings were more obvious than those of the WT. The differential expression of the related genes of photomorphogenesis in the mutant was much more than that in the WT. Three light and two JA responsive cis-affecting elements were identified during the promoter sequences of the AtPYL1 and AtPYL2 genes. These results suggested that functional polymorphism has evolved among the members of ZmPYL family. In response to developmental and environmental stimuli, they not only function as direct ABA receptors but also interact with components of other signaling pathways mediated JA, brassinosteroid(BR), auxin, etc., and even directly regulate downstream stress-related proteins. These signaling pathways can interact at various crosstalk points and different levels of gene expression within a sophisticated network. 相似文献